Evaluating Mosquitoes for Insecticide Resistance
Centers for Disease Control and Prevention
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 About this course

 Disease Vectors

 Strategies for Resistance Management

 Bottle Bioassay
 Larval Bioassay
 Microplate Assay
 Interpretation of Results

 Case Studies

 Links and resources

 Companion Materials

 (Glossary Explained)This term is defined in the glossary.

Bottle Bioassay

The bottle bioiassay allows resistance levels to be established for populations of mosquitoes reared in an insectary or collected in the field. The major advantages of the bottle assay are that any concentration of any insecticide may be evaluated at the one time. Secondly, the technique is simple and rapid.

Materials Needed (where to get materials)

  • 250 milliliter clean empty bottles with lids (one bottle for each insecticide sample and one bottle to function as control).*
  • acetone (reagent grade)
  • technical (analytical grade) or formulation grade insecticide
  • pipetors (one for each insecticide sample)
  • pipettor (one to be used for dosing each bottle with acetone)
  • aspirator
  • timer (digital counter capable of counting seconds)
  • Adult Mosquitoes (10-20 for each bottle)

    *Alternative size bottles can be used as long as they are all the same size for each test.


This procedure takes 5 steps. Steps are repeated for each insecticide formulation used.

Step 1:

Prepairing the formulation.

Step 2:

Coating the bottle.

Step 3:

Loading the mosquitoes in the bottles.

Step 4:

Timing and plotting mosquito mortality.

Step 5: Scoring the Data
Step 6: Clean up
  Beaker calibration (this should be known before begining)

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This page last reviewed July 3, 2001

Centers for Disease Control and Prevention
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