Evaluating Mosquitoes for Insecticide Resistance
Centers for Disease Control and Prevention
Help Glossary Quit    
2 of 6

 Last Step  Next Step

Site Contents


 About this course

 Disease Vectors

 Strategies for Resistance Management

 Bottle Bioassay
 Larval Bioassay
 Microplate Assay
 Interpretation of Results

 Case Studies

 Links and resources

 Companion Materials

 (Glossary Explained)This term is defined in the glossary.

Bottle Bioassay

  1. Dose the bottle with acetone using labeled pipette.
    Acetone is used to serve as a vehicle for spreading the insecticide over the bottle surface.
    Use the same amount for each bottle.
  1. Dose the bottle with prepared insecticide sample. (See table below for recommended dosages).
    Be sure to use a separate pipette to avoid cross contamination of different insecticide samples.
  1. Cap each bottle immediately to avoid premature evaporation.

Repeat this process for each insecticide sample.

On a sheet of graph paper, record the amount of insecticide you used on each bottle. Be sure to label each bottle with the type and concentration of insecticide it contains.

Dose a final bottle with acetone only to serve as control.

 Last Step  Next Step


Go to Top

| Help | Glossary | Quit | Previous | Menu | Next

| NCID Home | | CDC Home | Search | Health Topics A-Z |

This page last reviewed July 3, 2001

Centers for Disease Control and Prevention
National Center for Infectious Diseases

Privacy Policy | Accessibility