Evaluating Mosquitoes for Insecticide Resistance
Centers for Disease Control and Prevention
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 Disease Vectors

 Strategies for Resistance Management

 Bioassays
 Bottle Bioassay
 Larval Bioassay
 Microplate Assay
 Interpretation of Results

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 (Glossary Explained)This term is defined in the glossary.
Bottle Bioassay

Scoring data

Data is recorded at ten minute intervals. The first count should be made as soon as the mesquites are in the bottle.

Run four replicates of each insecticide plus one control (view sample run).

 

Mosquitoes are scored as alive or dead. It helps to gently rotate the bottle while counting.

Dead mosquitoes slide with the curvature of the bottle are more easily scored.

 

mortality study

Another animation will be used rather than photo above.

It is easier to count dead mosquitoes early in the test and live mosquitoes when few live ones remain.    
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This page last reviewed July 3, 2001

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