Evaluating Mosquitoes for Insecticide Resistance
Centers for Disease Control and Prevention
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 About this course

 Disease Vectors

 Strategies for Resistance Management

 Bottle Bioassay
 Larval Bioassay
 Microplate Assay
 Interpretation of Results

 Case Studies

 Links and resources

 Companion Materials

 (Glossary Explained)This term is defined in the glossary.

Establishing the Diagnostic Dosage

Doses are established for both the Bottle Bioassay and the Larval Bioassay the same way.

Assays are run on susceptible mosquitoes with a series of insecticide concentrations.

The data are plotted, creating a line graph similar to that in Figure 1. Convert the absolute mortality numbers to percents so that you will have consistent graphs that can be compared more easily.

Up to a point, the higher the concentration of insecticide used in an assay, the shorter length of time it takes for the insects to die. There is a concentration at or above which mosquitoes die at a maximal rate. The lowest dosage at which this occurs is the optimal diagnostic dosage.

This dosage should be used in all further assays with that particular insecticide and mosquito species.

Figure 1


  • The diagnostic dosage is the most useful dosage for detecting resistance.
  • A dose that is too low will result in false detection of resistance.
  • A dose that is too high will conceal resistance at some level.
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This page last reviewed July 3, 2001

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