Evaluating Mosquitoes for Insecticide Resistance
Centers for Disease Control and Prevention
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 About this course

 Disease Vectors

 Strategies for Resistance Management

 Bottle Bioassay
 Larval Bioassay
 Microplate Assay
 Interpretation of Results

 Case Studies

 Links and resources

 Companion Materials

 (Glossary Explained)This term is defined in the glossary.
Larval Bioassay

The larval bioiassay allows resistance levels to be established for populations of larval reared in an breading pan or collected in the field. The major advantages of the bottle assay are that any concentration of any insecticide may be evaluated at the one time. Secondly, the technique is simple and rapid.

Materials Needed: (where to get materials)

  • 150 ml glass beakers
  • wide-mouth pipette (used to transfer larve to beakers)
  • pipetors and tips (one for each insecticide sample)
  • Absolute alcohol
  • Chemical(s) to be tested
  • Stirring rod or similar equipment
  • Disposable plastic or wooden probes (toothpicks)
  • timer (digital counter capable of counting seconds)
  • Forceps or tweezers
  • Late 3rd or early 4th Instar Mosquito larvae


This procedure takes 4 steps. Steps are repeated for each insecticide forumation used.

Step 1:


Step 2:

Dosing the beakers

Step 3: Running the assay
Step 4: Clean up
  Beaker calibration (this should be known before begining)
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This page last reviewed July 3, 2001

Centers for Disease Control and Prevention
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