Larval Bioassay
The larval bioiassay allows resistance levels to be established for
populations of larval reared in an breading pan or collected in the field.
The major advantages of the bottle assay are that any concentration of
any insecticide may be evaluated at the one time. Secondly, the technique
is simple and rapid.
Materials Needed: (where to get materials)
- 150 ml glass beakers
- wide-mouth pipette (used to transfer larve to beakers)
- pipetors and tips (one for each insecticide sample)
- Absolute alcohol
- Chemical(s) to be tested
- Stirring rod or similar equipment
- Disposable plastic or wooden probes (toothpicks)
- timer (digital counter capable of counting seconds)
- Forceps or tweezers
- Late 3rd or early 4th Instar Mosquito larvae
Overview:
This procedure takes 4 steps. Steps are repeated for each insecticide
forumation used.
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